The product is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'?3' polymerase activity of Taq DNA polymerase, but also blocks the 5'?3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.
In addition, this product has been processed with the UCF.METM ultra-low residual process from Yeasen Biotechnology, resulting in extremely low residual levels of nucleases and host gDNA. With an optimized amplificationbuffer (e.g., Cat#16716ES), it can effectively reduce non-specificamplification caused by primer-probe hybridization during sample mixing, system warming, and long-term storage. This supports the development of a real time PCR system that allows for pre-mixing of primers and probes.