BlasTaq™ 2X qPCR MasterMix

Mfr.No.

G891

Description

BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains BlasTaq™ DNA Polymerase, a next-generation Taq polymerase strategically engineered by abm to deliver faster extension rates and stronger overall performance.

With optimized reaction conditions, BlasTaq™ DNA Polymerase offers significantly improved processivity, higher yields, and enhanced sensitivity. These improvements allow for faster reaction times, shortening qPCR runs by up to 70% compared to reactions using wild-type Taq polymerase. BlasTaq™ possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but lacks 3’-5’ exonuclease activity. It also produces 3’-dA-tailed amplicons, making qPCR products generated with BlasTaq™ compatible with TA cloning systems.

The MasterMix contains a fluorescent dye compatible with commonly used detection systems, such as SYBR Green™ and EvaGreen™. Additionally, ROX Reference Dye is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the ROX Machine Compatibility Chart.

Product Features:

Enable streamlined protocol in a simple reaction set-up
Allow accurate quantification of a variety of gene targets
Reduce pipetting steps to minimize the risk of contamination
Compatible with most real-time PCR instruments
Contains dye comparable to SYBR Green™ and EvaGreen™ (Probe-based qPCR Master Mix also available)
Included in abm's PCR Buffet Program

Template

DNA

Concentration

Detection Chemistry

BrightGreen

Format

MasterMix

Mix Concentration

Product Components

Product Component	Quantity
BlasTaq™ 2X qPCR MasterMix	500 rxn (4 x 1.25 ml)
ROX Reference Dye	50 µl

Quantity

4 x 1.25 ml for 500 reactions (20 µl)

Reaction

qPCR

Size

500 rxn (4 x 1.25 ml)

Concentration

Storage Condition

-20C

Shipping Condition

Dry Ice

Note

BlasTaq™ 2X qPCR MasterMix includes a separate vial of ROX Reference Dye. You can add this dye if your specific qPCR machine requires ROX, as indicated in the table below:

MACHINE TYPE	MACHINE
High ROX Machines	ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
	Biometra TOptical
	Fluidigm BioMark™
	Wafergene SmartChip System
	TianLong TL998 System
Low ROX Machines	ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
	BioGene InSyte™
	Illumina Eco
	Analytikjena qTower Series
	Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines	BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
	Roche LightCycler® (480, 1536, Nano)
	MJ Research Opticon™, Opticon™ 2, Chromo® 4
	Eppendorf™ Realplex 4
	BioGene SynChron™
	Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
	Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
	Cepheid SmartCyler, GeneXpert
	Enigma™ ML
	Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
	Qiagen Rotor-Gene™ (Q, 6000)
	Takara Dice™
	Thermo Scientific PikoReal
	DNA-Technology DT96, DTlite, DT-322
	Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
	Bioneer Exicycler™