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Home / PCR, qPCR & RT-PCR

OneScript Plus Reverse Transcriptase (PCR) + BlasTaq 2X PCR MasterMix

SKU : BHR10900098

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Applied Biological Materials (abm) Inc.

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Mfr.No.	G281
Description	Convenient and time-saving reaction set-up with abm’s EasyScript™ Two-Step RT-PCR Kit. This highly efficient two-step RT-PCR kit consists of two main components: EasyScript™ cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye. abm’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, abm’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. abm’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, abm offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. abm’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Key Features: Streamlined protocol suitable for high-throughput applications Ease of use with a simple set-up Excellent cDNA yield and robust PCR performance
Template	RNA
Trade Marker	OneScript®
Concentration	EasyScript™ (200 U/µl), 2X Reaction Mix, 2X PCR MasterMix
Format	Kit
Mix Concentration	2X
Quantity	25 reactions
Reaction	Towstep RTPCR

Reference	Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.

Concentration	EasyScript™ (200 U/µl), 2X Reaction Mix, 2X PCR MasterMix
Storage Condition	-20C
Shipping Condition	Dry Ice

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SKU	Supplier No.	Size	Your price	Quantity
BHR10900098	G281	25reactions	$55		Add to Cart

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