OneScriptPlus cDNA Synthesis Kit

Mfr.No.	G236
Description	Choose your priming methods with abm’s cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers. Key Features • Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers • Robust cDNA synthesis from any RNA template • High reproducibility and excellent yield This kit employs abm's EasyScript™ Plus Reverse Transcriptase .
Template	RNA
Trade Marker	OneScript®
Format	Kit
Quantity	100 x 20 µl reactions
Reaction	RT-PCR

Reference	Amodio, G et al. "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." Cell Bio. Toxicol. 285: (2016). DOI: 10.1007/s10565-016-9335-z. Antony, A et al. "MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration." Nat. Comm. : (2016). DOI: 10.1038/ncomms10955. Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Diana, D et al. "Inhibition of autocrine signaling of the Epithelial-Mesenchymal Transition in breast cancer." : (2016). Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Juskeviciute, E et al. "Inhibition of miR-21 rescues liver regeneration after partial hepatectomy in ethanol-fed rats." Amer. J. Physiol.-Gastrointestinal and Livery Physiology 5:G794-G806 (0). DOI: 10.1152/ajpgi.00292.2016 . Lakshmi , K et al. "A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration." BMC Genomics 1:1 (2016). DOI: 10.1186/s12864-016-2492-x. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Maher, SK et al. "Rethinking the biological relationships of the thyroid hormones, l-thyroxine and 3,5,3′-triiodothyronine." Comp. Biochem. Physiol. D: Genomics Proteomics :44-53 (2016). DOI: http://dx.doi.org/10.1016/j.cbd.2016.04.002. Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice." Aging Cell. 13(3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription. Park, H-S et al. "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." Cell Death Diff. :45-57 (2016). DOI: http://dx.doi.org/10.1016/j.bcp.2016.03.018. Song, JJ et al. "Casein glycomacropeptide‐derived peptide IPPKKNQDKTE ameliorates high glucose‐induced insulin resistance in HepG2 cells via activation of AMPK signaling." Mol. Nutr. Food Res. 2: (2017). DOI: 10.1002/mnfr.201600301. Application: RT-PCR. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.