Choose your priming methods with abm’s cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Key Features
• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield
This kit employs abm's EasyScript™ Plus Reverse Transcriptase .
Template
RNA
Trade Marker
OneScript®
Concentration
200 U/ul
Format
Kit
Quantity
25 x 20 µl reactions
Reaction
RT-PCR
Reference
Amodio, G et al. "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." Cell Bio. Toxicol. 285: (2016). DOI: 10.1007/s10565-016-9335-z.
Antony, A et al. "MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration." Nat. Comm. : (2016). DOI: 10.1038/ncomms10955.
Cavdar, Z et al. "Protective effects of taurine against renal ischemia/reperfusion injury in rats by inhibition of gelatinases, MMP-2 and MMP-9, and p38 mitogen-activated protein kinase signaling." Biotechnic & Histochemistry :1-12 (2017). DOI: 10.1080/10520295.2017.1367033. Application: RT-PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Diana, D et al. "Inhibition of autocrine signaling of the Epithelial-Mesenchymal Transition in breast cancer." : (2016).
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Juskeviciute, E et al. "Inhibition of miR-21 rescues liver regeneration after partial hepatectomy in ethanol-fed rats." Amer. J. Physiol.-Gastrointestinal and Livery Physiology 5:G794-G806 (2016). DOI: 10.1152/ajpgi.00292.2016 .
Lakshmi , K et al. "A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration." BMC Genomics 1:1 (2016). DOI: 10.1186/s12864-016-2492-x.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Maher, S.K et al. "Rethinking the biological relationships of the thyroid hormones, l-thyroxine and 3,5,3′-triiodothyronine." Comp. Biochem. Physiol. D: Genomics Proteomics :44-53 (0). DOI: http://dx.doi.org/10.1016/j.cbd.2016.04.002.
Park, H-S et al. "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." Cell Death Diff. : (0). DOI: doi: 10.1038/cdd.2016.6.
Serce , CU et al. "First Report of Grapevine Roditis leaf discoloration-associated virus Infecting Grapevine (Vitis vinifera) in Turkey." Plant Disease 1:256 (2018). DOI: 10.1094/PDIS-06-17-0927-PDN. Application: PCR.
Song, JJ et al. "Casein glycomacropeptide‐derived peptide IPPKKNQDKTE ameliorates high glucose‐induced insulin resistance in HepG2 cells via activation of AMPK signaling." Mol. Nutr. Food Res. 2: (2017). DOI: 10.1002/mnfr.201600301. Application: RT-PCR.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomed Pharmacother. 68(5):641-8 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: RT-PCR.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Zhu, J et al. "miR-19 targeting of GSK3β mediates sulforaphane suppression of lung cancer stem cells." The Journal of Nutritional Biochemistry :80-91 (2017). DOI: 10.1016/j.jnutbio.2017.02.020. Application: PCR.
Zhu, J et al. "Wnt/β-catenin pathway mediates (−)-Epigallocatechin-3-gallate (EGCG) inhibition of lung cancer stem cells." Biochem. Biophys. Res. Commun. 1:15-21 (2017). DOI: 10.1016/j.bbrc.2016.11.038. Application: PCR.