Lyophilized Bst Plus DNA Polymerase (60 U/uL, Glycerol free)

Mfr.No.

14405ES97, 14405ES60

Description

Bst Plus DNA Polymerase is derived from Bacillus stearothermophilus DNA Polymerase I. The 5 '-3' exonuclease activity was removed by genetic engineering method, but the 5 '-3' DNA polymerase activity and strong chain replacement activity were retained. Compared with wild-type Bst DNA Polymerase, the polymerase showed great improvement in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It was very suitable for isothermal amplification reactions such as LAMP reaction. This product freeze-dried glycerin-free Bst Plus DNA Polymerase to solve the problem of glycerinase-free instability and could be used to make freeze-dried products

Features

High ampliﬁcation eﬃciency
High dUTP tolerance
The freeze-dried product form

Product Components

Components No.	Name	14405ES60 (12 KU)	14405ES97 (120 KU)	14402ES98 (1,200 KU)
14405-A	Lyophilized Bst Plus DNA Polymerase (60 U/μL，Glycerol-Free)	1 Vial（200 μL）	5 Vials(400 μL)	10 Vials(2 mL)
14405-B	Lyophilized monoenzyme redissolution solution	200 μL	2 mL	20 mL

Specifications

Polymerase	Bst DNA Polymerase
Heat Inactivation	Incubation at 85°C for 5 min.
Activity Definition	1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.

Applications

Loop-mediated Isothermal Amplification (LAMP)

Application

Isothermal DNA amplification

Storage	The product should be stored at -25℃ ~ -15℃ for 1 years. Please avoid light.

Manual	Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800041-1	14405ES97	120 KU	$2105		Add to Cart
BHR20800041-2	14405ES60	12 KU	$225		Add to Cart

Lyophilized Bst Plus DNA Polymerase (60 U/uL, Glycerol free)

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