The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Buffer
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control
5μl of IRIS9 Plus Prestained Protein Ladder (PMI09) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. Stable for up to 24 months at -20°C.