It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg2, etc. The PCR-Sure™ Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H2O with the PCR-Sure™ 2X MasterMix.
Each Reaction Mix contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
Template
DNA
Description
*There are a total of 12 Individual Reaction Mixes. After the optimal reaction condition is identified, the Individual Reaction Mixes may be ordered separately. For the catalog number G065-X, the "X" indicates a number from 1 to 12. When ordering, please specify a unique catalog number.
Format
Mix
Quantity
200 reactions
Reaction
PCR
Reference
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.