Hifair^TM AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)

Hifair™ AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) is an upgraded version of Hifair™ ? 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus). Compared to the previous generation Hifair™ ? Reverse Transcriptase, this product exhibits enhanced tolerance to inhibitors, faster reverse transcription speed, and higher cDNA yield, making it suitable for reverse transcription reactions of RNA from animals, plants, and microorganisms. The gDNA Digester Mix -V2 included in this product has been significantly improved in its ability to remove residual DNA, effectively digesting up to 1000 ng of DNA, ensuring more reliable results by eliminating DNA contamination in RNA templates.

Hifair™ AdvanceFast SuperMix contains all components required for reverse transcription. Simply add RNA template and RNase-free H₂O to initiate the reaction, while simultaneously terminating the activity of gDNA Digester to ensure cDNA integrity.

The reverse transcription products are suitable for dye-based and probe-based qPCR. However, they are not recommended for long-fragment amplification such as gene cloning. For such applications, Hifair™ AdvanceFast 1st Strand cDNA Synthesis Kit (Cat#11150ES) is recommended for high-efficiency reverse transcription.

Specifications

Components

Storage

This product should be stored at -25~-15? for 1 years.

Instructions

1. When DNA Removal is Required

1) DNA Digestion

Prepare the following mixture in an RNase-free microcentrifuge tube, mix gently by pipetting, and incubate at 37? for 2 min:

*It is recommended to use ? 2 µg of Total RNA. If the target gene expression is low, the amount can be increased up to 5 µg of Total RNA.

2) Reverse Transcription Reaction Setup (20 µL System):

3) Reaction Program:

2. When DNA Removal is Not Required

1) Reverse Transcription Reaction Setup (20 µL System):

*It is recommended to use ? 2 µg of Total RNA. If the target gene expression is low, the amount can be increased up to 5 µg of Total RNA.

2) Reaction Program:

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves?for your safety.

3. Perform all pipetting and mixing steps on ice to maintain sample integrity.

4. Gently invert and centrifuge each component at low speed before use to ensure proper mixing.

Document:

 Manual