Hieff Unicon^TM qPCR TaqMan Probe Master Mix

Mfr.No.

11205ES08, 11205ES03

Description

Hifair^TM Advanced One Step RT-qPCR SYBR Green Kit is a kit for fluorescence quantification based on SYBR Green I dye. Using gene-specific primers, reverse transcription and qPCR reactions are completed in one tube, eliminating the need for repeated cap-opening and pipetting operations, greatly improving assay efficiency and reducing the risk of contamination. For RNA samples, the kit employs heat-resistant Hifair^TM V Reverse Transcriptase for efficient cDNA synthesis and UNICON^TM HotStart Taq DNA Polymerase for quantitative amplification. Under the optimized buffer system, the sensitivity of the kit can be as high as 0.1 pg for highly expressed targets and as high as 1 pg for moderately expressed targets, and the kit is also suitable for amplification and quantification of DNA samples. The kit is suitable for the amplification and quantification of DNA samples. It enables the sensitive detection and quantification of nucleic acids from different plant and animal samples, cells and microorganisms.

Features

High specificity
High sensitivity

Product Components

Components No.	Name	11205ES03	11205ES08	11205ES25
11205-A	Hieff Unicon™ qPCR TaqMan Probe Master Mix	1 mL	5×1 mL	25 mL
11205-B	50×Low Rox	40 μL	200 μL	1 mL
11205-C	50×High Rox	40 μL	200 μL	1 mL

Specifications

Hot Start	Built-in hot start
Detection method	Primer-probe detection
PCR method	RT-qPCR
Polymerase	Taq DNA polymerase
Type of sample	RNA
Application equipment	Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne™ Plus; 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6, 7, 12k Flex; Bio-Rad：CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf：Mastercycler ep realplex, realplex 2 s; Qiagen：Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science：LightCycler 480, LightCycler 2.0; Lightcycle r 96; Thermo Scientific：PikoReal Cycler; Cepheid：SmartCycler; Illumina：Eco qPCR.

Applications

Probe-Based qPCR

Reference	[1] Huang T, Li X, Wang F, et al. The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy. Cell Death Dis. 2021;12(4):333. Published 2021 Mar 29. doi:10.1038/s41419-021-03629-4(IF:8.469) [2] Ma H, Lin J, Li L, et al. Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis. Chemosphere. 2021;282:131149. doi:10.1016/j.chemosphere.2021.131149(IF:7.086) [3] Lu L, Li X, Zhong Z, et al. KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy. Int J Biol Sci. 2021;17(15):4093-4107. Published 2021 Oct 3. doi:10.7150/ijbs.62867(IF:6.582) [4] Lu L, Zhong Z, Gu J, Nan K, Zhu M, Miao C. ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy. Mol Med. 2021;27(1):74. Published 2021 Jul 8. doi:10.1186/s10020-021-00339-7(IF:6.354) [5] Li X, Lu L, Hou W, et al. The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy. J Transl Med. 2022;20(1):147. Published 2022 Mar 29. doi:10.1186/s12967-022-03352-4(IF:5.531) [6] Shen X, Chen X, Wang J, et al. SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN. Exp Mol Med. 2020;52(10):1715-1729. doi:10.1038/s12276-020-00509-3(IF:5.418) [7] Qi J, Chen X, Wu Q, et al. Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression. Oxid Med Cell Longev. 2020;2020:3985089. Published 2020 Mar 19. doi:10.1155/2020/3985089(IF:5.076) [8] Zeng W, Ren J, Li Z, et al. Levistolide A Inhibits PEDV Replication via Inducing ROS Generation. Viruses. 2022;14(2):258. Published 2022 Jan 27. doi:10.3390/v14020258(IF:5.048) [9] Li D, Ma H, Shu Q, et al. Arsenite inhibits M2a polarization of macrophages through downregulation of peroxisome proliferator-activated receptor gamma [published online ahead of print, 2022 Jun 28]. Toxicol Appl Pharmacol. 2022;450:116142. doi:10.1016/j.taap.2022.116142(IF:4.460) [10] Long J, Liu L, Zhou X, Lu X, Qin L. HLA-DQB1-AS1 Promotes Cell Proliferation, Inhibits Apoptosis, and Binds with ZRANB2 Protein in Hepatocellular Carcinoma. J Oncol. 2022;2022:7130634. Published 2022 May 11. doi:10.1155/2022/7130634(IF:4.375) [11] Hou W, Lu L, Li X, Sun M, Zhu M, Miao C. c-Myc participates in high glucose-mediated endothelial inflammation via upregulation of IRAK1 expression in diabetic nephropathy. Cell Signal. 2022;92:110263. doi:10.1016/j.cellsig.2022.110263(IF:4.315) [12] Ma H, Ding Z, Xie Y, et al. Methylglyoxal produced by tumor cells through formaldehyde-enhanced Warburg effect potentiated polarization of tumor-associated macrophages. Toxicol Appl Pharmacol. 2022;438:115910. doi:10.1016/j.taap.2022.115910(IF:4.219) [13] Cao N, Tang Z, Zhang X, et al. Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1. Front Vet Sci. 2022;8:792322. Published 2022 Feb 8. doi:10.3389/fvets.2021.792322(IF:3.412) [14] Qi J, Wu Q, Cheng Q, Chen X, Zhu M, Miao C. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. J Diabetes Res. 2020;2020:2308520. Published 2020 Feb 23. doi:10.1155/2020/2308520(IF:2.965)

Storage	The product should be stored at -25°C~-15℃ away from light for 18 months. Please avoid repeated freeze-thaw.

Manual	Safety Data Sheet Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800017-1	11205ES08	5mL	$205		Add to Cart
BHR20800017-2	11205ES03	1 mL	$65		Add to Cart

Hieff Unicon^TM qPCR TaqMan Probe Master Mix

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