All-In-One 5X RT MasterMix with gDNA Removal

Mfr.No.

G592

Description

The All-In-One 5X RT MasterMix with gDNA Removal offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, all in a single tube. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis.

The optimized MasterMix includes abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo(dT)s and Random Primers. The OneScript® Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for enhanced cDNA yield and integrity. By eliminating the need for multiple separate reagents, this all-in-one mix offers a straightforward, reliable, and reproducible workflow that consistently delivers high-quality cDNA, even from challenging or low-quality RNA samples—ideal for a wide range of downstream molecular biology applications.

Product Features:

Easy one-step setup reduces pipetting and sample handling
Removes contaminating gDNA with temperature-sensitive DNase
High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G138)
Also includes dNTPs, Oligo (dT)s, and random primers
Included in abm's PCR Buffet Program

Template

RNA

Concentration

Format

MasterMix

Product Components

Product Component	Quantity
All-In-One 5X RT MasterMix	100 rxn (400 µl)
Nuclease-Free H2O	1.0 ml

Quantity

100 x 20 µl reactions

Reaction

RT-PCR

Size

100 rxn

Application

Generation of templates for use in RT-PCR and qRT-PCR
cDNA synthesis from ssRNA
cDNA library construction
Generation of probes for hybridization
DNA primer extension

Reference

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Concentration	5X
Storage Condition	-20C
Shipping Condition	Dry Ice
Note	For applications using pure RNA where genomic DNA removal is not required, try our OneScript® Hot 5X RT MasterMix (Cat. No. G590). It offers a faster protocol for an even more efficient cDNA synthesis.
Storage	Store all components at -20C

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All-In-One 5X RT MasterMix with gDNA Removal

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