From RNA template to cDNA in just 15 minutes completely hassle-free.Withabm’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Key Features • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield
Note- In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Literature
Quantity
1000 x 10 µl reactions
Reference
Yu,et al. "Effects of ten–eleven translocation 1 (Tet1) on DNA methylation and gene expression in chicken primordial germ cells." Reproduction, Fertility, and Development : (2018).DOI: 10.1071/RD18145. Application: RT-PCR.
Lu, Jet al. "Alliin attenuates 1, 3-dichloro-2-propanol-induced lipogenesis in HepG2 cells through activation of the AMP-activated protein kinase-dependent." Life Sciences 195:19-24 (2018).DOI: 10.1016/j.lfs.2017.12.040. Application: PCR.
Wang, Wet al. "The late embryogenesis abundant gene family in tea plant (Camellia sinensis): Genome-wide characterization and expression analysis in response to cold and dehydration stress." Plant Physiology and Biochemistry 135:277-286 (2018).DOI: 10.1016/j.plaphy.2018.12.009. Application: RT.
Bradley, Ret al. "Glucagon-like peptide-2 requires a full-complement of Bmi-1 for its proliferative effects in the murine small intestine." Endocrinology 7: (2016).DOI: http://dx.doi.org/10.1210/en.2016-1127. Application: Reverse Transcription.
Hanson, Met al. "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." BioMed Central 1:228 (2016).DOI: 10.1186/s12862-016-0805-y. Application: Reverse Transcription.
Zhou, Het al. "LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies." Frontiers in microbiology :166 (2016).DOI: 10.3389/fmicb.2016.00166. Application: Reverse Transcription.
Zhou, Het al. "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." Scientific reports :25545 (2016).DOI: 10.1038/srep25545. Application: Reverse Transcription.
Li, Let al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016).DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription.
Deng, Het al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016).DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription.
Tuerdi, Net al. "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." Nanomed. Nanotechnol. Biol. Med. 7:1899-1907 (2016).DOI: http://dx.doi.org/10.1016/j.nano.2016.05.002. Application: Reverse Transcription.
Su, Xet al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016).DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription.
Liu, J.Cet al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." International Journal of Cardiology :388-393 (2016).DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription.
Sun, Met al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016).DOI: 10.1111/cei.12896. Application: Reverse Transcription.
Yang, Set al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription.
Deng, Het al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016).DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription.
Liu, Fet al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016).DOI: 10.1111/nph.14021. Application: Reverse Transcription.
Jeon, H.Jet al. "Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation." Int. Biodeterior. Biodegrad. :202-208 (2016).DOI: http://dx.doi.org/10.1016/j.ibiod.2016.06.012. Application: Reverse Transcription.
Unsoy, Get al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014).DOI: 10.1016/j.biopha.2014.04.003.PubMed: 24880680. Application: Reverse Transcription.
Chung, Get al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011).DOI: 10.1016/j.dnarep.2011.09.011.PubMed: 21968058. Application: PCR.
Lee, Get al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012).DOI: 10.5772/1268. Application: Reverse Transcription.
Jones, MRet al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012).DOI: 10.1007/s00438-012-0681-0.PubMed: 22350747. Application: PCR.