Nanobody-Tn5

Mfr.No.

12595ES01, 12595ES03

Description

Nanobody-Tn5 is a recombinant Tn5 transposase fused with nanobody which can specific bind with primary antibodies from two different species (mouse and rabbit).

Product Information

Catalog No.	12595ES01/12595ES02/12595ES03
specifications	10 μL/ 80 μL/ 100 μL

Product Components

Component No.	Component name	12595ES01	12595ES02	12595ES03
12595-A	Anti-mouse Tn5	10 μL	80 μL	100 μL
12595-B	Anti-rabbit Tn5	10 μL	80 μL	100 μL
12595-C	Assemble buffer	10 μL	80 μL	100 μL

H3K4me1:

H3K4me3

H3K9me3

H3K27ac

H3K27me3

Control: Conventional pA/G-Tn5, Cut&Tag Multi Cut&Tag: Nanobody Tn5
Instruction
Take high-throughput sequencing library preparation as an example, be sure to read the instructions carefully before use.
1. Adapter preparation
1) Reference oligo name and sequence of Illumina platform:
Oligo A-1：5'-AATGATACGGCGACCACCGAGATCTACACATAAGGCTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3'
Oligo A-2：5'-AATGATACGGCGACCACCGAGATCTACACATATCCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3'
Oligo A-3：5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′
Oligo B：5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′
Oligo C：5'-phos-CTGTCTCTTATACACATCT-3'
2) Dissolve the Oligo A, Oligo B, and Oligo C to 200 μM.
3) The following reaction systems are formulated respectively:

component	Reaction 1
Oligo B （200 μM）	10 μL
Oligo C （200 μM）	10 μL
total	20 μL

component	Reaction 2
Oligo A-1 or A-2 or A-3（200 μM）	10 μL
Oligo C （200 μM）	10 μL
total	20 μL

4) Mix reaction 1 and 2 together, and briefly centrifuge to bring the solution back to the bottom of the tube. Place in the PCR instrument, set up the following program and run it: hot lid at 105°C,and then heat at 94℃ for 2 min. After the time was up, the reaction procedure was stopped without opening the hot lid.The PCR tube was naturally cooled in the PCR instrument for 2 h, and then placed at 4℃ for 5 min.
5) After the reaction, mix the equal volumes of Reaction 1 and Reaction 2. Named ad Adapter Mix and stored from – 25 to – 15℃.
2. for transposon generation
1) To configure the following reaction system:

component	volume (μL)
Anti-mouse Tn5 or Anti-rabbit Tn5	10
Adapter mix (50 μM)	0.5
Assemble Buffer	0.5

2) Reaction conditions: use the pipette to gently blow and mix well. Perform the reaction at 25℃ for 1 h (hot lid off). The products of reaction can be directly applied to the library preparation or stored from -25 to -15℃.

Notes

1. Transposase is temperature-sensitive, so it is recommended to complete the adapter embedding as soon as possible. The generated transposon can be stored at-25~ -15℃, with a validity period of 1 year.
2. This product is used for scientific research purposes only.
3. For your safety and health, please wear laboratory clothes and wear disposable gloves.

Storage	Stored at -85° ～ -65℃ for one year.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHS20800025-1	12595ES01	10 uL	$365		Add to Cart
BHS20800025-2	12595ES03	100 uL	$3250		Add to Cart

Nanobody-Tn5

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