Hieff NGS^TM FFPE DNA Repair Reagent

Hieff NGS^TM FFPE DNA Repair Reagent is specifically designed for FFPE samples to repair varying degrees of damage in low-quality FFPE DNA, enhancing library yield and sequencing quality. This product can be directly used with Yeasen Hieff NGS^TM Library Preparation Kits (Cat#12197/Cat# 12927) and the A-tailing module (Cat# 12608), with compatible systems that can repair 5 ng to 1 ?g of fragmented FFPE DNA. Additionally, this product is also suitable for use with Yeasen Fragmentation Enzyme-based Library Preparation Kit.

Features

High Repair Efficiency: Provides higher repair efficiency for FFPE DNA.
Streamlined Workflow: Simple operation with strong adaptability.
High Compatibility: Enzyme components can be used with the end-repair and A-tailing system of library preparation kits, requiring only a single buffer to complete FFPE DNA repair and end-repair/dA-tailing.

Applications

Repair of damaged low-quality FFPE samples

Library preparation for low-quality FFPE samples

Specifications

Components

Shipping and Storage

This product should be stored at -25~-15? for 1 year.

Figures

Figure1. The impact of FFPE repair reagents on library yield

During the library construction process, two sets of experiments were conducted to compare the same samples. One group included a repair reagent in the end-repair and A-tailing step, while the other group did not. The experimental results showed that the FFPE repair reagent significantly increased library yield for low-quality FFPE samples by repairing the samples, whereas its effect on high-quality FFPE samples was less pronounced.                                                       

Documents:

Safety Data Sheet

12606_MSDS_HB250208_EN.PDF

Manuals

12606_Manual_Ver.EN20250206.pdf

Citations & References: