Activity: 2000 units per milligram of papain before immobilization Loading: 10mg papain/g of settled gel
Intended Use
FuncS
Relevance
Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.
Species
Purified from papaya latex
Application
For research use only
Form
Liquid
Nature
Native
Protein Length
Full length protein
Research Area
Cell Biology
Target
Immobilized Papain
Purity
>95%(SDS-PAGE)
Storage
Aliquot and store at -20? or -80?. Avoid repeated freeze/thaw cycles.