Sample Size: 15.0 uL per testX-Reactivity within panel : @ 10,000 pg/mLIGF-I negligable with Primate IGF-IIIGF-II minimal (<1%) with Primate IGF-IIntra Assay CV:<10% Inter Assay CV<20%Recovery 70-130% standard dose
Description
Multi-Plex ( 2 Plex) Flow Cytometry Assay Panel- components to measure non-human Primate: Insulin-LikeGrowth Factor IGF-I and IGF-II simultaneously- in PrimateSerum, Plasma, Cell Culture Supernatant or Cell Lysatesamples.
Assay Type
SUPER-X PLEXTM FLOW CYTOMETRY CYTOKINE ASSAYS
Caution
Contains Sodium Azide - ( 0.05%) - Dispose contentscarefully, flush with water to avoid reaction with lead drainlines.
Notes
Universal Sample Diluent Kit is available for thePrimate IGF-I IGF-II panel ( Universal for all sampletypes) Order cat# DKIGF2 (Diluent Kit)Cell Lysate Prep Buffer (CLP200) or Sample Dilution Buffer( to dilute high concentration samples) -(SDL200)also available.
Sample Availability
Note: Serum and Plasma samples, and other sample types mayneed pre-treatment to release IGF-I and IGF-II for accuratemeasurement. An acid-ethanol extraction method described by: Breier,et al, ( J. Endocrinology; 1991; V128; 347-357) may be useful.Appropriate pre-treatment method for sample type and experimentalconditions should be determined by investigator.
Standard Solution
One vial of lyophilized standard containingPre-mixed multiplexed analytes ( 2-Plex) ( see vial insert) -single standard vial can be used for one or more assaysindicated.