Sensitivity:< 50.0 pg/mL / Sample Size: 15.0 uL per testRange: 100.0 - 20,000 pg/mLIntra Assay CV:<10% Inter Assay CV<20%Recovery 70-130% standard dose
Description
Flow Cytometry Assay components to measure humanTGF beta-1 in Human, Mouse, Rat Canine, Porcine or nonhumanPrimate samples.
Assay Type
SUPER-X PLEXTM FLOW CYTOMETRY CYTOKINE ASSAYS
Caution
Contains Sodium Azide - ( 0.05%) - Dispose contentscarefully, flush with water to avoid reaction with lead drainlines.
Notes
Samples should be pre-treated with Hcl, thenneutralized for activation of latent TGF Beta-1. ( DO NOTpre-treat the TGF beta standard). Add 20uL of HCl (1N)to 100 uL sample ( mix). Incubate 10 min. @ RT Neutralize with added 20 uL of 1.2N NaOH/0.5MHEPESActivated sample may need to be diluted with Sample DilutionBuffer ( cat# SDL200). Dilution factor determined byinvestigator.Due to pre-treatment of samples, this assay NOT recommendedto be multi-plexed with other Super-X Plex assays.
Standard Solution
One vial of lyophilized, recombinant TGF Beta-1 standard