Sensitivity:< 30.0 pg/mL / Sample Size: 15.0 uL per testRange: 80.0 - 20,000 pg/mLIntra Assay CV:<10% Inter Assay CV<20%Recovery 70-130% standard dose
Description
Flow Cytometry Assay components to measure humanLatent TGF beta-1 ( also known as LAP- LatencyAssociated protein) in Human samples.
Assay Type
SUPER-X PLEXTM FLOW CYTOMETRY CYTOKINE ASSAYS
Caution
Contains Sodium Azide - ( 0.05%) - Dispose contentscarefully, flush with water to avoid reaction with lead drainlines.
Notes
This assay detects the latent TGF Beta complex targetedto the extracellular matrix. As a result, NO PRE-TREATMENTof samples are required for measurement of the Latent TGFBeta and can be multi-plexed with other assaysOrder the matching ANTIGENIX Starter Kit (STKH10)And Sample Diluent Kit ( to match sample type):Human Serum/Plasma (DKH100)orCell Culture Supernatant (DKC100) orCell Lysate (DKL100)Cell Lysate Prep Buffer (CLP200) or Sample Dilution Buffer( to dilute high concentration samples) -(SDL200)also available
Standard Solution
One vial of lyophilized, recombinant latent TGF Beta-1complex standard ( LAP).
Storage
Store Refrigerated ( 4-8° C.)
Reference
Justin P. Annes, John Munger, D. Rifkin;Making Sense of latent TGF beta Activation;Journal Cell Science; 116: 217-224 ( 2003).