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Home / Assay Kits / Multiplex Immunoassay

EcoPlexTM Human T Helper Cytokine 14-Plex Panel Kit

SKU : BHE10309339

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	MPH009
Description	This kit is for detection of GM-CSF, IFN-gamma, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNF-alpha in human serum, plasma, biological fluid and cell culture supernatant.
Reactivity	Human
Abbreviation	GM-CSF, IFN-gamma, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNF-alpha
Assay Time	3h
Important Notes	Cross-reactivity of analytes in the Panel?Negligible
Precision	Intra-assay CV<10% Inter-assay CV<20%
Recovery	70%-130%
Sensitivity	GM-CSF<5pg/mL TNF-alpha<1pg/mL IL-2<3pg/mL IFN-gamma<3pg/mL IL-9<5pg/mL IL-12p70<3pg/mL IL-4<1pg/mL IL-5<2pg/mL IL-6<5pg/mL IL-17A<3pg/mL IL-22<3pg/mL IL-21<5pg/mL IL-10<2pg/mL IL-13<5pg/mL
Test Principle	EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Uniprot	GM-CSF:P04141///TNF-alpha:P01375///IL-2:P60568///IFN-gamma:P01579///IL-9:P15248///IL-12p70:P29459&P29460///IL-4:P05112///IL-5:P05113///IL-6:P05231///IL-17A:Q16552///IL-22:Q9GZX6///IL-21:Q9HBE4///IL-10:P22301///IL-13:P35225

Shipping	ice bag
Storage	The kit can be stored at 2?8°C for up to 12 months by protecting the kit from light.

Product Manual	Manual MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHE10309339	MPH009	96T	$4615		Add to Cart

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