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Home / Assay Kits / Multiplex Immunoassay

EcoPlexTM Human Inflammation 10-Plex Panel Kit

SKU : BHE10309333

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	MPH003
Description	This kit is for detection of IFN-gamma, IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IL-18, MCP-1 and TNF-alpha in human serum, plasma, biological fluid and cell culture supernatant.
Reactivity	Human
Abbreviation	IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IL-18, MCP-1, and TNFalpha
Assay Time	3h
Important Notes	Cross-reactivity of analytes in the Panel?Negligible
Precision	Intra-assay CV<10% Inter-assay CV<20%
Recovery	70%-130%
Sensitivity	IL-1beta<5pg/mL IL-10<2pg/mL IL-1alpha<2pg/mL TNF-alpha<1pg/mL IFN-gamma<3pg/mL IL-12p70<3pg/mL IL-8<1pg/mL IL-6<5pg/mL IL-18<5pg/mL MCP-1<5pg/mL
Test Principle	EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Uniprot	IL-1beta:P01584///IL-10:P22301///IL-1alpha:P01583///TNF-alpha:P01375///IFN-gamma:P01579///IL-12p70:P29459&P29460///IL-8:P10145///IL-6:P05231///IL-18:Q14116///MCP-1:P13500

Shipping	ice bag
Storage	The kit can be stored at 2?8°C for up to 12 months by protecting the kit from light.

Product Manual	Manual MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHE10309333	MPH003	96T	$3305		Add to Cart

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