14-3-3 protein zeta/delta is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Amino acids LLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQ from the human protein were used as the immunogen for the YWHAZ antibody.
Isotype
IgG2b
Predicted Reactivity
Human, Mouse, Rat, Monkey
Reactivity
Human, Mouse, Rat, Monkey
Recombinant
No
Antigen
14-3-3 zeta/delta
Uniprot
P63104
Clone No
6G5
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the YWHAZ antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
FACS, WB
Dilution
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells