Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and the two proteins exhibit a high level of sequence identity (greater than 95%).
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Human vinculin purified from uterus was used as the immunogen for this Vinculin antibody.
Isotype
IgG1
Predicted Reactivity
Human, mouse, rat, chicken
Reactivity
Rat, Chicken, Human, Mouse
Recombinant
No
Antigen
Vinculin
Gene Id
7414
Clone No
HVIN-1
Format
Ascites
Purification
Unpurified ascites
Storage
After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-F, WB, IF
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (Frozen): 2-4ug/ml for formalin or acetone fixed tissues,Immunofluorescence: 2-4ug/ml