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Home / Primary Antibodies / Monoclonal Antibodies

Vimentin antibody

SKU : BHA10809385

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Mfr.No.	FNab09410
Alternative Names	FLJ36605 ; VIM ; Vimentin
Background	Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Categories	Primary Antibodies
Cellular Localization	Cytoplasm, Intermediate Filament
Clonality	monoclonal
Description	Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Host	Mouse
Immunogen	vimentin
Isotype	IgG1
Molecular Weight	55 kDa
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Subcellular Location	Cytoplasm, Intermediate filament
Synonyms	FLJ36605, VIM, Vimentin

Uniprot	P08670
Clone No	2G10
Research Area	Developmental biology, Immunology, Cancer, Stem Cells, Neuroscience
Weight	55kDa

Form	liquid
Format	liquid
Purification	Protein A+G purification
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

Applications

ELISA, WB

Description

ELISA ; WB

Dilution

WB	1:1000-1:10000
IHC	1:20-1:2000
IF	1:20-1:200

Tested Application

WB: 1:1000-1:10000; IHC: 1:200-1:2000; IF: 1:20-1:200

Manual	FNab09410.pdf

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SKU	Supplier No.	Size	Your price	Quantity
BHA10809385	FNab09410	100ug	$300		Add to Cart

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