The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. The Vimentin gene expresses in human lymphocytes and in Burkitts lymphoma cells and it is secreted by activated macrophages. The gene encoding human protein is located on the short arm of chromosome 10.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Pig eye lens vimentin was used as the immunogen for this Vimentin antibody.
Isotype
IgG1
Predicted Reactivity
Human, rabbit, pig, rat
Reactivity
Pig, Rat, Human, Rabbit
Recombinant
No
Antigen
Vimentin
Gene Id
7431
Clone No
V9
Format
Ascites
Purification
Unpurified ascites
Storage
After reconstitution, the Vimentin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB, IF
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1.5-2.5ug/ml,Immunofluorescence: 2-4ug/ml