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Home / Primary Antibodies / Monoclonal Antibodies

VIM Antibody / Vimentin

SKU : BHA17127946

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NSJ Bioreagents

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Mfr.No.	V9098IHC, V9098IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Human recombinant protein was used as the immunogen for this VIM antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the antibody should be determined by the researcher.

Uniprot	P08670

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the VIM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/vim-antibody-spm576-v9098" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17127946	V9098IHC, V9098IHC-7ML	7ml	$539		Add to Cart

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