Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Host
Mouse
Immunogen
Purified recombinant Human UCHL1 protein fragments expressed in E.coli.
Isotype
IgG2b
Raised In
Mouse
Reactivity
Human
Regulatory
RUO
Relevance
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Species
Homo Sapiens (Human)
Antigen
UCHL1 Monoclonal
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Form
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Format
liquid
Purification
Affinity purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB
Description
ELISA ; WB, WB ; ELISA
Dilution
WB
1:500-1:5000
Tested Application
ELISA,WB;Recommended dilution:WB:1:500-1:5000
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Reviews of UCHL1 Monoclonal Antibody (CSB-MA868921)