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Home / Primary Antibodies / Monoclonal Antibodies

Tyrosinase Antibody

SKU : BHA17127939

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NSJ Bioreagents

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Mfr.No.	V9093IHC, V9093IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant tyrosinase protein was used as the immunogen for this Tyrosinase antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the antibody should be determined by the researcher.

Uniprot	P14679

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/tyrosinase-antibody-spm360-v9093" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17127939	V9093IHC, V9093IHC-7ML	7ml	$539		Add to Cart

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