SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Amino acids MLLLADMDVVNQLVAGGQFRVVKEPL from the human protein were used as the immunogen for the Synaptophysin antibody.
Isotype
IgG2b
Predicted Reactivity
Mouse, Rat
Reactivity
Rat, Mouse
Recombinant
No
Antigen
Synaptophysin
Uniprot
P08247
Clone No
3G12
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Purified
Purification
Affinity purified
Storage
After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.