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Home / Primary Antibodies / Monoclonal Antibodies

Recombinant TP53 Antibody (N-Terminal Region)

SKU : BHA17125390

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NSJ Bioreagents

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Mfr.No.	V3647SAF, V3647SAF-100UG
Clonality	Recombinant Mouse Monoclonal
Description	This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 5 1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human wild-type p53 protein was used as the immunogen for this recombinant TP53 antibody. The epitope has been mapped to within amino acids 16-25.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant TP53 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the recombinant TP53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125390	V3647SAF, V3647SAF-100UG	100ug	$539		Add to Cart

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