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Home / Primary Antibodies / Monoclonal Antibodies

Recombinant B2M Antibody / Beta-2 Microglobulin

SKU : BHA17114167

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NSJ Bioreagents

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Mfr.No.	V3539, V3539-20UG, V3539-100UG
Clonality	Monoclonal
Description	Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Rabbit
Immunogen Region	Full length recombinant human protein was used as the immunogen for the recombinant B2M antibody.
Isotype	IgG,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	Yes
Subcellular Location	Cytoplasmic

Antigen	Beta-2 Microglobulin
Uniprot	P61769
Clone No	B2M/1857R

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein A affinity chromatography
Storage	Store the recombinant B2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IHC-P, WB, FACS, IF
Dilution	Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Product Manual	Data Sheet

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