F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids Y97-R124) was used as the immunogen for the Prothrombin antibody.
Isotype
Rabbit IgG
Reactivity
Human
Recombinant
No
Antigen
Prothrombin
Uniprot
P00734
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml