This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.
Isotype
IgG
Predicted Reactivity
All Species
Reactivity
All Species
Recombinant
Yes
Antigen
Phosphotyrosine
Clone No
RM111
Concentration
1 mg/ml
Format
Purified
Purification
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant Phosphotyrosine antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Applications
WB, IP, ICC, IHC, ChIP
Dilution
Western blot: 1:1000 -1:5000,Immunoprecipitation: 1:200-1:1000,Chromatin IP (ChIP): 1:200-1:1000,Immunocytochemistry: 1:200-1:500,Immunohistochemistry: 1:200-1:500 (1)