EIF2S1 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. (UniProt)
Formulation
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Host
Rabbit
Immunogen Region
A synthetic peptide specific to human EIF2S1 (surrounding phosphorylated S51) was used as the immunogen for the phospho-EIF2S1 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
Phospho-EIF2S1
Uniprot
P05198
Clone No
IO-5
Format
Purified
Purification
Affinity purified
Storage
Store the phospho-EIF2S1 antibody (pS51) at -20°C.
Applications
IHC-P, WB
Dilution
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200