ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Host
Mouse
Immunogen
Purified recombinant Human ICAM1(C-term.) protein fragments expressed in E.coli.
Isotype
IgG2a
Raised In
Mouse
Reactivity
Human
Regulatory
RUO
Relevance
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Species
Homo Sapiens (Human)
Antigen
ICAM1 Monoclonal
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Form
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Format
liquid
Purification
Affinity purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB
Description
ELISA ; WB, WB ; ELISA
Dilution
WB
1:500-1:5000
Tested Application
ELISA,WB;Recommended dilution:WB:1:500-1:5000
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Reviews of ICAM1 Monoclonal Antibody (CSB-MA947664)