HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
A human recombinant protein (amino acids M1-K205) was used as the immunogen for the Hsp27 antibody.
Isotype
IgG1
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
HSP27
Uniprot
P04792
Clone No
3H3
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the Hsp27 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
FACS, ICC/IF, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunocytochemistry/Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells