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Home / Primary Antibodies / Monoclonal Antibodies

Golgi Marker Antibody / Golgi apparatus protein 1 / GLG1

SKU : BHA17112138

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NSJ Bioreagents

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Mfr.No.	V2543, V2543-20UG, V2543-100UG
Clonality	Monoclonal
Description	This mAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This mAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Golgi complex in cytoplasm

Antigen	Golgi
Uniprot	Q92896
Clone No	GLG1/970

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the Golgi Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IHC-P
Dilution	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Product Manual	Data Sheet

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