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Home / Primary Antibodies / Monoclonal Antibodies

GFAP Antibody Cocktail

SKU : BHA17123944

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NSJ Bioreagents

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Mfr.No.	V2529SAF, V2529SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	GFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the GFAP antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P14136

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the GFAP antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123944	V2529SAF, V2529SAF-100UG	100ug	$539		Add to Cart

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