Glial fibrillary acidic protein is an intermediate filament protein of 52kDa. Its gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in the protein are associated with Alexander disease.
Host
Rabbit
Immunogen Region
Glial fibrillary acidic protein from pig spinal cord was used as the immunogen for this GFAP antibody.
Isotype
Rabbit IgG
Reactivity
Rat, Mouse, Human
Recombinant
No
Antigen
GFAP
Concentration
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the GFAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
IF, IHC-F, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.4-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml (formalin or acetone fixed tissues),Immunofluorescence: 2-4ug/ml