GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
GAP-43 from neonatal rat forebrain membranes was used as the immunogen for this GAP43 antibody.
Isotype
IgG2a
Predicted Reactivity
Human, mouse, rat
Reactivity
mouse, rat, Human
Recombinant
No
Antigen
GAP43
Gene Id
2596
Clone No
GAP-7B10
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the GAP43 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-F, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml--formalin or acetone fixed tissues