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Home / Primary Antibodies / Monoclonal Antibodies

Fascin Antibody

SKU : BHA17126068

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Mfr.No.	V7155SAF, V7155SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Full length recombinant human protein was used as the immunogen for the Fascin antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the Fascin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q16658

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Fascin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IF, IHC-P, FACS
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126068	V7155SAF, V7155SAF-100UG	100ug	$539		Add to Cart

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