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Home / Primary Antibodies / Monoclonal Antibodies

Cytokeratin 7 Antibody

SKU : BHA17124131

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NSJ Bioreagents

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Mfr.No.	V2657IHC, V2657IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P08729

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124131	V2657IHC, V2657IHC-7ML	7ml	$539		Add to Cart

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