It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
The ovarian carcinoma cell line OTN 11 was used as the immunogen for the Cytokeratin 7 antibody.
Isotype
kappa, Mouse IgG1
Species Reactivity
Human
Note
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.
Uniprot
P08729
Format
Biotin Conjugate
Purity
Protein G affinity chromatography
Storage
Store the Cytokeratin 7 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Applications
WB, FACS, IF, IHC-P
Description
Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood,Immunofluorescence: 2-4ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml at RT