COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Human Collagen IV recombinant protein (amino acids G1445-T1669) was used as the immunogen for the Collagen IV antibody.
Isotype
IgG1
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Secreted
Antigen
Collagen IV
Uniprot
P02462
Clone No
3G3
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Purified
Purification
Protein G affinity
Storage
After reconstitution, the Collagen IV antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml