Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. It is mapped to 11p11.2. This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interact ernatively spliced transcript variants encoding different isoforms have been identified.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
A human recombinant partial protein (amino acids M1-L221) was used as the immunogen for the CKAP5 antibody.
Isotype
IgG2b
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Subcellular Location
Cytoplasmic
Antigen
Ch-TOG
Uniprot
Q14008
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the CKAP5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, IF, WB, FACS
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells