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Home / Primary Antibodies / Monoclonal Antibodies

CD99 Antibody

SKU : BHA17124223

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NSJ Bioreagents

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Mfr.No.	V2707IHC, V2707IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This mAb shows a very similar reactivity to other CD99 mAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
Isotype	Mouse IgM, kappa
Species Reactivity	Human
Note	Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P14209

Format	Purified
Purity	PEG precipitation
Storage	Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124223	V2707IHC, V2707IHC-7ML	7ml	$539		Add to Cart

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