CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: (T,gp55). CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
CD4-transfected mouse T-cell hybridoma (3DT) followed by CD4+ human T-cell CEM cells was used as the immunogen for this CD4 antibody.
Isotype
IgG1
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
CD4
Gene Id
920
Clone No
CA-4
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the CD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-F, FACS
Dilution
Immunohistochemistry (Frozen): 1ug/ml-formalin or acetone fixed tissues,Flow cytometry: 1-3ug/million cells