The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Human thymocytes followed by Sezary T cells was used as the immunogen for this CD3 antibody.
Isotype
IgG1
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
CD3
Gene Id
916
Clone No
CA-3
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the CD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-F, FACS
Dilution
Immunohistochemistry (Frozen): 1ug/ml-formalin or acetone fixed tissues,Flow cytometry: 1-3ug/million cells