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Home / Primary Antibodies / Monoclonal Antibodies

CD20 Antibody

SKU : BHA17123357

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Mfr.No.	V2048SAF, V2048SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CD20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	Functional studies, FACS, IF
Description	Functional studies (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123357	V2048SAF, V2048SAF-100UG	100ug	$539		Add to Cart

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