The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Amino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.
Isotype
IgG1
Predicted Reactivity
Human, mouse, rat
Reactivity
mouse, rat, Human
Recombinant
No
Antigen
Beta-Actin
Gene Id
60
Clone No
AC-15
Format
Ascites
Purification
Unpurified ascites
Storage
After reconstitution, the beta-Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml