Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Amino acids Q22-M119 from the human protein were used as the immunogen for the B2M antibody.
Isotype
IgG2b
Predicted Reactivity
Human, Monkey
Reactivity
Human, Monkey
Recombinant
No
Antigen
Beta-2 Microglobulin
Uniprot
P61769
Clone No
2H10
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Purified
Purification
Protein G affinity
Storage
After reconstitution, the B2M antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IF, FACS, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence: 2-4ug/ml